教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Twist Controls Skeletal Development and Dorsoventral Patterning by Regulating Runx2 in Zebrafish
學年100
學期1
出版(發表)日期2011/11/01
作品名稱Twist Controls Skeletal Development and Dorsoventral Patterning by Regulating Runx2 in Zebrafish
作品名稱(其他語言)
著者Yang, Der-Chih; Tsai, Chih-Chien; Liao, Yun-Feng; Fu, Hui-Chuan; Tsay, Huey-Jen; Huang, Tung-Fu; Chen, Yau-Hung; Hung, Shih-Chieh
單位淡江大學化學學系
出版者San Francisco: Public Library of Science
著錄名稱、卷期、頁數PLoS One 6(11), e27324(9pages)
摘要Background: Twist1a and twist1b are the principal components of twists that negatively regulate a number of cellular signaling events. Expression of runx2 and downstream targets is essential for skeletal development and ventral organizer formation and specification in early vertebrate embryos, but what controls ventral activity of maternal runx2 and how twists function in zebrafish embryogenesis still remain unclear.
Methodology/Principal Findings: By studying the loss of twist induced by injection of morpholino-oligonucleotide in zebrafish, we found that twist1a and twist1b, but not twist2 or twist3, were required for proper skeletal development and dorsoventral patterning in early embryos. Overexpression of twist1a or twist1b following mRNA injection resulted in deteriorated skeletal development and formation of typical dorsalized embryos, whereas knockdown of twist1a and twist1b led to the formation of abnormal embryos with enhanced skeletal formation and typical ventralized patterning. Overexpression of twist1a or twist1b decreased the expression of runx2b, whereas twist1a and twist1b knockdown increased runx2b expression. We have further demonstrated that phenotypes induced by twist1a and twist1b knockdown were rescued by runx2b knockdown.
Conclusions/Significance: Together, these results suggest that twist1a and twist1b control skeletal development and dorsoventral patterning by regulating runx2b in zebrafish and provide potential targets for the treatment of diseases or syndromes associated with decreased skeletal development.
關鍵字
語言英文
ISSN1932-6203
期刊性質國外
收錄於SCI
產學合作
通訊作者Chen, Yau-Hung; Hung, Shih-Chieh
審稿制度
國別美國
公開徵稿
出版型式紙本
相關連結
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