教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Hypoxia Inhibits Osteogenesis in Human Mesenchymal Stem Cells through Direct Regulation of RUNX2 by TWIST
學年100
學期1
出版(發表)日期2011/09/01
作品名稱Hypoxia Inhibits Osteogenesis in Human Mesenchymal Stem Cells through Direct Regulation of RUNX2 by TWIST
作品名稱(其他語言)
著者Yang, Der-Chih; Yang, Muh-Hwa; Tsai, Chih-Chien; Huang, Tung-Fu; Chen, Yau-Hung; Hung, Shih-Chieh
單位淡江大學化學學系
出版者San Francisco: Public Library of Science
著錄名稱、卷期、頁數PLoS ONE 6(9), e2365(10pages)
摘要Background: Bone loss induced by hypoxia is associated with various pathophysiological conditions, however, little is known about the effects of hypoxia and related signaling pathways on osteoblast differentiation and bone formation. Because bone marrow-derived mesenchymal stem cells (MSCs) survive under hypoxic conditions and readily differentiate into osteoblasts by standard induction protocols, they are a good in vitro model to study the effects of hypoxia on osteoblast differentiation.
Methodology/Principle Findings: Using human MSCs, we discovered TWIST, a downstream target of HIF-1α;, was induced under hypoxia and acted as a transcription repressor of RUNX2 through binding to the E-box located on the promoter of type 1 RUNX2. Suppression of type 1 RUNX2 by TWIST under hypoxia further inhibited the expression of BMP2, type 2 RUNX2 and downstream targets of RUNX2 in MSCs.
Conclusions/Significance: Our findings point to the important role of hypoxia-mediated signalling in osteogenic differentiation in MSCs through direct regulation of RUNX2 by TWIST, and provide a method for modifying MSC osteogenesis upon application of these cells in fracture healing and bone reconstruction.
關鍵字
語言英文
ISSN1932-6203
期刊性質國外
收錄於SCI;
產學合作
通訊作者Chen, Yau-Hung; Hung, Shih-Chieh
審稿制度
國別美國
公開徵稿
出版型式,電子版
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