教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the
Suppression of Myeloperoxidase Expression
學年99
學期2
出版(發表)日期2011/07/01
作品名稱Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the
Suppression of Myeloperoxidase Expression
作品名稱(其他語言)
著者Liao, Yun-Feng; Chiou, Min-Chuan; Tsai, Jen-Ning; Wen, Chi-Chung; Wang, Yun-Hsin; Cheng, Chien-Chung; Chen, Yau-Hung
單位淡江大學數學學系; 淡江大學化學學系
出版者臺北市行政院衛生署藥物食品檢驗局
著錄名稱、卷期、頁數Journal of Food and Drug Analysis=藥物食品分析 19(2), pp.167-173
摘要Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line “Tg (mpx:GFP)” expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level.
關鍵字cyclooxygenase; inflammation; myeloperoxidase; resveratrol; zebrafish
語言英文
ISSN1021-9498
期刊性質國外
收錄於SCI
產學合作
通訊作者Cheng, Chien-Chung; Chen, Yau-Hung
審稿制度
國別中華民國
公開徵稿
出版型式紙本
相關連結
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