教師資料查詢 | 類別: 期刊論文 | 教師: 陳銘凱 CHERN MING-KAI (瀏覽個人網頁)

標題:Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2
學年97
學期1
出版(發表)日期2008/08/01
作品名稱Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2
作品名稱(其他語言)
著者Wang, Shih-juei; Yu, Chi-yang; Lee, Cheng-kang; Chern, Ming-kai; Kuan, I-ching
單位淡江大學生命科學研究所
出版者Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數Biotechnology Letters 30(8), pp.1415-1422
摘要D-Amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis (RtDAO and TvDAO) are both yeast homodimeric flavoenzymes. Two of their cDNA genes were connected by a hexanucleotide linker and heterologously expressed in E. coli to produce the corresponding double DAOs (dRtDAO and dTvDAO) with two subunits fused into a single polypeptide. The specific activities of double DAOs remained similar to those of native dimeric DAOs, although the catalytic efficiencies (kcat/KM) were decreased due to higher KM values. The Tm value for dRtDAO was shifted 5°C higher while that for dTvDAO was increased only by 2°C, in comparison with the corresponding native counterparts. In the presence of 10 mM H2O2, dRtDAO and dTvDAO exhibited half-lives of about 60 and 40 min, respectively, which were 2- and 1.5-fold, respectively, longer than their native DAOs. These yeast DAOs can therefore be thermally and oxidatively stabilized by linking their subunits together.
關鍵字D-Amino acid oxidase; Gene fusion; Rhodosporidium toruloides; Stability; Trigonopsis variabilis
語言英文
ISSN0141-5492; 1573-6776
期刊性質國外
收錄於SCI
產學合作
通訊作者Kuan, I-ching
審稿制度
國別荷蘭
公開徵稿
出版型式紙本;電子版
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