Marchantin A, a cyclic bis(bibenzyl ether), isolated from the liverwort Marchantia emarginata subsp.tosana, induces apoptosis in human MCF-7 breast cancer cells
學年 98
學期 2
出版（發表）日期 2010-05-01
作品名稱 Marchantin A, a cyclic bis(bibenzyl ether), isolated from the liverwort Marchantia emarginata subsp.tosana, induces apoptosis in human MCF-7 breast cancer cells
作品名稱（其他語言）
著者 Huang, Wei-jan; Wu, Chia-li; Lin, Chia-wei; Chi, Li-ling; Chen, Pen-yuan; Chiu, Chun-jung; Huang, Chung-yang; Chen, Chia-nan
單位 淡江大學化學學系
出版者 Shannon: Elsevier Ireland Ltd
著錄名稱、卷期、頁數 Cancer Letters 291(1), pp.108-119
摘要 Liverwort constituents have been reported to exert a broad spectrum of biological activities. In this study, we used a bioactivity-guided separation of an extract from the liverwort species Marchantia emarginata subsp. tosana to determine its anticancer activity. A high level of the active ingredient was isolated from this liverwort and its chemical structure was identified and characterized by various spectra. It was found to be identical to a well-known compound, marchantin A, a cyclic bisbibenzyl ether. However, no anticancer activities of this compound have previously been reported. We found that marchantin A efficiently induced cell growth inhibition in human MCF-7 breast cancer cells, with an IC50 of 4.0 μg/mL. Fluorescence microscopy and a Western blot analysis indicated that marchantin A actively induced apoptosis of MCF-7 cells. The levels of cleaved caspase-8, cleaved caspase-3, cleaved caspase-9, and cleaved poly (ADP ribose) polymerase (PARP) increased. However, the level of Bid markedly decreased in a dose- and time-dependent manner. We also evaluated the anticancer activities of marchantin A on the regulation of cell cycle regulators such as p21, p27, cyclin B1, and cyclin D1. The p21 and p27 gene expressions increased markedly while cyclin B1 and D1 gene expression decreased markedly by treatment with marchantin A. Many report demonstrated that liverwort was suggested to possess potent antioxidant activity. Our results indicate that marchantin A possesses free radical-scavenging activity (EC50 = 20 μg/mL). Taken together, for the first time, the compound marchantin A from liverworts demonstrated to be a potent inducer of apoptosis in MCF-7 cells.
關鍵字 Marchantia tosana; Marchantin A; Breast cancer; Apoptosis; Cyclic bis(bibenzyl ether)
語言 en
ISSN 0304-3835
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chen, Chia-nan
審稿制度
國別 IRL
公開徵稿
出版型式 紙本
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