教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Development of a whole-organism model to screen new compounds for sun protection
學年97
學期2
出版(發表)日期2009/06/01
作品名稱Development of a whole-organism model to screen new compounds for sun protection
作品名稱(其他語言)
著者Wang, Yun-hsin; Wen, Chi-chung; Yang, Zhi-shiang; Cheng, Chien-chung; Tsai, Jen-ning; Ku, Chia-chen; Wu, Hsin-ju; 陳曜鴻; Chen, Yau-hung
單位淡江大學化學學系; 淡江大學數學學系
出版者Springer
著錄名稱、卷期、頁數Marine Biotechnology 11(3), pp.419-429
摘要We used zebrafish as a whole-organism model to screen new compounds for sun protection activity. First of all, we designed a series of UVB exposure experiments and recorded the phenotypic changes of zebrafish embryos. Results showed that 100 mJ/cm2 of UVB given six times separated by 30 min intervals is the best condition. Fin malformation (reduced and/or absent fin) phenotypes are the most evident consequences after exposure to UVB. Each fin was affected by UVB, including pelvic, ventral, caudal, and dorsal fin, but pelvic fin seemed to be the most sensitive target after UVB exposure. We furthermore carried out “prevention” and “treatment” experiments using green tea extract and/or (−)-epigallocatechin (EGCG) to test this whole-organism model by observing the morphological changes of all fins (especially pelvic fin) after UVB exposure. Effects of UVB, green tea extract and EGCG on fin development were assessed using the Kaplan–Meier analysis, log-rank test and Cox proportional hazards regression. Results showed that a zebrafish pelvic fin in the UVB + green tea (treatment) group is 5.51 (range from 2.39 to 14.90) times, one in the UVB + green tea (prevention) group is 7.04 (range from 3.11 to 18.92) times, and one in the 25 ppm of EGCG (prevention) group is 22.19 (range from 9.40 to 61.50) times more likely to return to normal fin than one in the UVB only group. On the basis of these observations, we believe this model is effective for screening the higher stability and lower toxicity of new compounds, such as small chemicals which are derivative from EGCG or other dietary agents for sun protection.
關鍵字EGCG; Fin; Tea; UV; Zebrafish
語言英文
ISSN1436-2228
期刊性質國內
收錄於
產學合作
通訊作者
審稿制度
國別
公開徵稿
出版型式
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!