教師資料查詢 | 類別: 期刊論文 | 教師: 曾琇瑱 Tseng Shio-jenn (瀏覽個人網頁)

標題:Electrophoretic behaviors of human hepatoma HepG2 cells
學年97
學期2
出版(發表)日期2009/05/01
作品名稱Electrophoretic behaviors of human hepatoma HepG2 cells
作品名稱(其他語言)
著者Hsu, Jyh-Ping; Ku, Ming-Hong; Yang, Li-Chia; Lu, Jui-Nan; Young, Tai-Horng H.; Tseng, Shio-Jenn
單位淡江大學數學學系
出版者Weinheim: Wiley - V C H Verlag GmbH &; Co. KGaA
著錄名稱、卷期、頁數Electrophoresis 30(9), pp.1531-1537
摘要The electrophoretic mobility of HepG2 cells was measured and a charge-regulated model was proposed to simulate the results obtained. Here, a cell was simulated by a rigid core and an ion-penetrable membrane layer containing both acidic and basic functional groups. The influences of the key parameters, including the pH, the ionic strength, the thickness of the membrane layer of a cell, the density and the dissociation constant of the dissociable functional groups in the membrane layer, and the binding constant of divalent cations on the electrophoretic mobility of a cell were investigated. In particular, the role of the buffer used in the experiment was discussed; this effect was neglected in almost all the relevant theoretical analyses in the literature. We showed that the binding ability of divalent cations to the dissociated functional groups in the membrane layer of a cell ranks as Ca2+>Mg2+>hexamethonium.
關鍵字Buffer effect; Electrophoretic mobility; HepG2 cells; Ion-binding; Surface dissociation reaction
語言英文
ISSN1522-2683
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者
審稿制度
國別德國
公開徵稿
出版型式電子版
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