教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment
學年97
學期2
出版(發表)日期2009/05/26
作品名稱Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment
作品名稱(其他語言)
著者Wang, San-lang; Wu, Pei-Chen; Liang, Tzu-wen
單位淡江大學生命科學研究所
出版者Kidlington: Pergamon
著錄名稱、卷期、頁數Carbohydrate Research, 344(8), pp.979-984
摘要We have developed a culture system for efficient production of chitosanase by Bacillus sp. TKU004. TKU004 was cultivated by using squid pen powder as the sole carbon/nitrogen source. The effects of autoclave treatments of the medium on the production of chitosanase were investigated. Autoclave treatment of squid pen powder for 45 min remarkably promoted enzyme productivity. When the culture medium containing an initial squid pen powder concentration of 3% was autoclaved for 45 min, the chitosanase activity was optimal and reached 0.14–0.16 U/mL. In addition, extracellular surfactant-stable chitosanase was purified from the TKU004 culture supernatant. The antioxidant activity of TKU004 culture supernatant was determined through the scavenging ability of DPPH, with 70% per mL. With this method, we have shown that marine wastes can be utilized efficiently through prolonged autoclave treatments to generate a high value-added product, and have revealed its hidden potential in the production of functional foods.
關鍵字Chitosanase;Antioxidant;Squid pen wastes;Bacillus sp.;Autoclave treatment
語言英文
ISSN0008-6215
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者Wang, San-lang
審稿制度
國別英國
公開徵稿
出版型式紙本
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