教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Conversion and degradation of shellfish wastes by Bacillus cereus TKU018 fermentation for the production of chitosanase and bioactive materials
學年98
學期1
出版(發表)日期2009/12/15
作品名稱Conversion and degradation of shellfish wastes by Bacillus cereus TKU018 fermentation for the production of chitosanase and bioactive materials
作品名稱(其他語言)
著者Wang, San-Lang; Chen, Tz-Rung; Liang, Tzu-Wen; Wu, Pei-Chen
單位淡江大學化學系
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Biochemical Engineering Journal 48(1), pp.111-117
摘要Two chitosanases (CHSB1 and CHSB2) were purified from the culture supernatant of Bacillus cereus TKU018 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of CHSB1 and CHSB2 determined by SDS–PAGE were approximately 44 kDa and 22 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSB1 and CHSB2 were (pH 5, 60 °C; pH 5–7, <40 °C) and (pH 7, 50 °C; pH 4–7, <50 °C), respectively. CHSB1 and CHSB2 were both inhibited by EDTA and CHSB1 was inhibited completely by 5 mM Zn2+. CHSB1 and CHSB2 degraded chitosan with DD ranging from 60% to 95%, but did not degrade chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU018 culture supernatant (1.5% SPP) incubated for 3–4 days has 75% relative antioxidant activity (DPPH scavenging ability). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials.
關鍵字Chitosanase;Antioxidant;Shrimp shell wastes;Squid pen wastes;Bacillus cereus;Reducing sugar
語言英文
ISSN1369-703X
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者Wang, San-Lang (sabulo@mail.tku.edu.tw)
審稿制度
國別NTD
公開徵稿
出版型式紙本
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