教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Foxd3 mediates zebrafish myf5 expression during early somitogenesis
學年94
學期2
出版(發表)日期2006/02/15
作品名稱Foxd3 mediates zebrafish myf5 expression during early somitogenesis
作品名稱(其他語言)
著者陳曜鴻; Chen, Yau-hung; Lee, H. C.; Huang, H. Y.; Lin, C. Y.; Tsai, H. J.
單位淡江大學生命科學研究所
出版者Elsevier
著錄名稱、卷期、頁數Developmental Biology 290(2), pp. 359-372
摘要Myf5, one of the basic helix–loop–helix transcription factors, controls muscle differentiation and is expressed in somites during early embryogenesis. However, the transcription factors bound to the cis-elements of myf5 are poorly understood. In this study, we used the yeast one-hybrid assay and found that Forkhead box d3 (Foxd3) interacted specifically with the −82/−62 cassette, a key element directing somite-specific expression of myf5. The dual-luciferase assay revealed that the expression of Foxd3 potently transactivated the myf5 promoter. Knocking down foxd3 with morpholino oligonucleotide (MO) resulted in a dramatic down-regulation of myf5 in somites and adaxial cells but not in the presomitic mesoderm. On the other hand, myod expression remained unchanged in foxd3 morphants. Foxd3 mediation of myf5 expression is stage-dependent, maintaining myf5 expression in the somites and adaxial cells during the 7- to 18-somite stage. Furthermore, in the pax3 morphant, the expression of foxd3 was down-regulated greatly and the expression of myf5 was similar to that of the foxd3 morphant. Co-injection of foxd3 mRNA and pax3-MO1 greatly restored the expression of myf5 in the somites and adaxial cells, suggesting that pax3 induces foxd3 expression, which then induces the expression of myf5. This report is the first study to show that Foxd3, a well-known regulator in neural crest development, is also involved in myf5 regulation.
關鍵字myf5;foxd3;pax3;Zebrafish;Myogenesis
語言英文(美國)
ISSN0012-1606
期刊性質國外
收錄於
產學合作
通訊作者
審稿制度
國別美國
公開徵稿
出版型式,紙本
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