Transgenic zebrafish line with over-expression of Hedgehog on the skin: a useful tool to screen Hedgehog-inhibiting compounds
學年 98
學期 1
出版（發表）日期 2009-12-01
作品名稱 Transgenic zebrafish line with over-expression of Hedgehog on the skin: a useful tool to screen Hedgehog-inhibiting compounds
作品名稱（其他語言）
著者 Chen, Yau-hung; Wang, Yun-hsin; Yu, Tsung-han; Wu, Hsin-ju; Pai, Chiung-wen
單位 淡江大學生命科學研究所
出版者 Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數 Transgenic Research 18(6), pp.855-864
摘要 We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histology revealed embryos derived from Tg(k18:shh:RFP) displayed an elevated Nuclear/Cytoplasmic ratio and pleomorphic nuclei compared to their wild type littermates, suggesting the abnormal growth pattern on the epidermis of Tg(k18:shh:RFP) embryos were dysplasia. Later (by 7 dpf), Tg(k18:shh:RFP) embryos displayed broader pectoral fins which are similar to the polydactyly phenotypes of Nevoid basal cell carcinoma syndrome (NBCCS)/Gorlin patients and polydactylous mice. In addition, treatment with cyclopamine is able to enhance and prolong the survival rates and survival durations of Tg(k18:shh:RFP) embryos. In conclusion, this unique Tg(k18:shh:RFP) fish line, should be an excellent experimental animal for screening for a lower toxicity level of the new Hh-inhibitor and can even be used as a new anti-cancer drug-screening platform.
關鍵字 Basal cell carcinoma; Keratin; Skin cancer; Sonic hedgehog; Transgenic fish; Zebrafish
語言 en
ISSN 0962-8819
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chen, Yau-hung
審稿制度
國別 NLD
公開徵稿
出版型式 紙本
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