教師資料查詢 | 類別: 期刊論文 | 教師: 余良杰 YU, LIANG-JYE (瀏覽個人網頁)

標題:A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA
學年92
學期1
出版(發表)日期2003/10/04
作品名稱A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA
作品名稱(其他語言)
著者Chang, C. C.; Wu, J. Y.; Chien, C. W.; Wu, W. S.; Liu, H.; Kang, C. C.; 余良杰; Yu, Liang-jye; Chang, D. C.
單位淡江大學化學學系
出版者American Chemical Society (ACS)
著錄名稱、卷期、頁數Analytical Chemistry 75(22), pp.6177-6183
摘要We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T2AG3)4. The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 μM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 μM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti−anti−anti−anti and anti−anti−syn−syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti−syn−anti−syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T2AG3)4 can be revealed by BMVC.
關鍵字
語言英文(美國)
ISSN0003-2700
期刊性質國外
收錄於
產學合作
通訊作者
審稿制度
國別美國
公開徵稿
出版型式,紙本
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