教師資料查詢 | 類別: 期刊論文 | 教師: 吳俊弘 Wu, Chunhung (瀏覽個人網頁)

標題:A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer
學年87
學期1
出版(發表)日期1998/08/02
作品名稱A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer
作品名稱(其他語言)
著者吳俊弘; Wu, Chunhung; Liu, Tianbo; Chu, Benjamin
單位淡江大學化學學系
出版者Elsevier
著錄名稱、卷期、頁數Journal of Non-Crystalline Solids 235-237, pp.605-611
摘要The unique sol–gel transition property of E99P69E99 (with E and P being poly(ethylene oxide) and poly(propylene oxide), respectively), a Pluronic polyol F127, could become a useful separation medium in the miniaturization and automation of capillary electrophoresis (CE). Static light scattering (SLS), dynamic light scattering (DLS), and small-angle X-ray scattering were used to study the solution property and the gel-like structures of F127 in a CE buffer (1X TBE) for DNA separation. F127 in the CE forms core-shell micelles with a closed-association process. The critical micelle concentration (cmc) decreases and the micellar association number increases with increasing temperature. At larger solution concentrations, small-angle X-ray scattering results showed that the gel-like structure formed by close packing of the micelles had a face centered cubic (fcc) lattice. In the gel-like region the association number and also the micellar size were not affected by concentration changes. With F127 solution as a separation medium, CE could be performed successfully with a detection length of only 4 cm for separation of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) fragments.
關鍵字C274;B125;R177
語言英文
ISSN0022-3093
期刊性質國外
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產學合作
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審稿制度
國別新加坡
公開徵稿
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