| A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer | |
|---|---|
| 學年 | 87 |
| 學期 | 1 |
| 出版（發表）日期 | 1998-08-02 |
| 作品名稱 | A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer |
| 作品名稱（其他語言） | |
| 著者 | 吳俊弘; Wu, Chunhung; Liu, Tianbo; Chu, Benjamin |
| 單位 | 淡江大學化學學系 |
| 出版者 | Elsevier |
| 著錄名稱、卷期、頁數 | Journal of Non-Crystalline Solids 235-237, pp.605-611 |
| 摘要 | The unique sol–gel transition property of E99P69E99 (with E and P being poly(ethylene oxide) and poly(propylene oxide), respectively), a Pluronic polyol F127, could become a useful separation medium in the miniaturization and automation of capillary electrophoresis (CE). Static light scattering (SLS), dynamic light scattering (DLS), and small-angle X-ray scattering were used to study the solution property and the gel-like structures of F127 in a CE buffer (1X TBE) for DNA separation. F127 in the CE forms core-shell micelles with a closed-association process. The critical micelle concentration (cmc) decreases and the micellar association number increases with increasing temperature. At larger solution concentrations, small-angle X-ray scattering results showed that the gel-like structure formed by close packing of the micelles had a face centered cubic (fcc) lattice. In the gel-like region the association number and also the micellar size were not affected by concentration changes. With F127 solution as a separation medium, CE could be performed successfully with a detection length of only 4 cm for separation of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) fragments. |
| 關鍵字 | C274;B125;R177 |
| 語言 | en |
| ISSN | 0022-3093 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | SGP |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/25178 ) |
GoogleAnalytics