期刊論文
| 學年 | 114 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2026-02-24 |
| 作品名稱 | From projection to true surface Curvature-corrected SEM morphometrics of diatom frustules under hydration variability |
| 作品名稱（其他語言） | |
| 著者 | Izabela Zglobicka; Maciej Łojkowski; EwaBorucinska; Przemysław Dąbek; Ewa Górecka; Yzu-Hung Ma; Yu-Po Chen; Mehmet Aladag; Jakub Augustyniak; Ping-Hung Yeh; Krzysztof J. Kurzydlowski |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Journal of Microscopy 302(2), p.210–220 |
| 摘要 | Diatom frustules exhibit highly organised silica architectures with submicron features, which are widely studied. Nowadays, scanning electron microscopy (SEM) is commonly used for studying their morphology. However, SEM provides a two-dimensional projection that distorts the true size of three-dimensional features. In addition, samples for SEM observations are usually prepared by removing organic parts and drying the remaining parts made of bio-silica (so-called frustule). Here, we combine high-resolution SEM and liquid-cell SEM imaging with confocal microscopy to construct a curvature-aware correction model that accounts for the surface area distortion in SEM imaging caused by the in-plane projection of three-dimensional frustule geometry, demonstrated using Diploneis didyma frustules as a case study. The paper describes how automated image analysis enabled the detection of individual pores, the localisation of their centroids, and the calculation of curvature-corrected surface areas. True diameters of the frustules are provided, and drying-induced shrinkage of pores is obtained. Species length-based normalisation of morphometric data reduced variability and allow to infer systematic dependence of pore sizes on position on frustules. The results establish a reliable framework for quantitative characterisation of diatom frustules and can be applied across disciplines requiring accurate morphometrics of biogenic silica structures. LAY DESCRIPTION: Curvature-corrected SEM morphometrics, paired with hydrated/dehydrated imaging, offer a reliable quantitative framework for diatom frustule pore-size assessment. Drying shrinks pores by ∼10%, so dry-sample measurements should be corrected when targeting living/wet morphology. Curvature correction compensates SEM projection distortion, with uncorrected pores underestimated by ∼34% on average, enabling realistic edge-to-centre comparisons. The protocol is broadly transferable beyond Diploneis didyma with minor adjustments and supports applications in taxonomy, biomonitoring, and bioinspired design. |
| 關鍵字 | SEM morphometrics; bioinspired materials; curvature correction; diatoms; hydration state |
| 語言 | en |
| ISSN | |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129645 ) |