期刊論文
| 學年 | 114 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2026-03-05 |
| 作品名稱 | Effects of antigen dosage and chitosan micro/nanoparticle size on immune responses in mice immunized with H5N1 influenza vaccine |
| 作品名稱（其他語言） | |
| 著者 | Nguyen AD; Nguyen YN; Pham H; Ha TDL; Nguyen HL; Le L; Nguyen VB; Nguyen DS; Dinh HH; Wang SL; Cao V |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Polymers 18(5), p.642 |
| 摘要 | Highly pathogenic avian influenza A/H5N1 remains a persistent threat to public health and poultry production. H5N1 antigens are typically poorly immunogenic and require effective adjuvants for antigen dose-sparing. Here, we evaluated chitosan microparticles (CSMs) and nanoparticles (CSNs) as polymeric nano-adjuvants for an H5N1 influenza vaccine, focusing on the roles of antigen dose and particle size. A purified hemagglutinin antigen was adsorbed onto chitosan particles at doses ranging from 0.15 to 5.0 µg. Both CSNs and CSMs showed consistently high loading efficiency (97–99%). BALB/c mice were immunized intramuscularly in a prime–boost schedule. Chitosan nanoparticles significantly enhanced IgG and hemagglutination inhibition (HI) titers at low antigen doses compared with aluminum hydroxide and antigen-only controls (p < 0.05). Immune responses reached saturation at a 1.5 µg dose of antigen for chitosan nanoparticles and 3.0 µg for chitosan microparticles. IgG subtype analysis suggested a balanced IgG1/IgG2a profile. Collectively, these findings support chitosan-based polymeric nanoparticles as promising adjuvants enabling dose-sparing H5N1 vaccination. |
| 關鍵字 | chitosan; nanoparticles; microparticles; polymeric adjuvant; H5N1 influenza vaccine; hemagglutination inhibition; antigen dose-sparing |
| 語言 | en |
| ISSN | 2073-4360 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Wang, San-Lang |
| 審稿制度 | 是 |
| 國別 | CHE |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/128715 ) |