期刊論文
| 學年 | 106 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2017-11-01 |
| 作品名稱 | Copper, Nickel, and Zinc Cations Biosorption Properties of Gram-positive and Gram-negative MerP Mercury-resistance Proteins |
| 作品名稱（其他語言） | |
| 著者 | Yi-Huang Hsueh, Kuen-Song Lin, Yu-Ting Wang, Chao-Lung Chiang |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Journal of the Taiwan Institute of Chemical Engineers 80, p.168-175 |
| 摘要 | The mercury resistance (mer) operon is present in a wide range of bacteria but relevant research has been conducted mainly on Gram-negative bacteria. We compared the heavy metal biosorption properties of Gram-positive and Gram-negative MerP, a key metal-binding transporter of the mer operon that scavenges heavy metal ions in the periplasm for subsequent neutralization in the cytoplasm by other Mer proteins. The MerP proteins of the Gram-positive Bacillus cereus RC607 and Gram-negative Pseudomonas aeruginosa K-62 strains in Escherichia coli strain BL21 were expressed to derive the recombinant strains BL21+MerPBc and BL21+MerPPa, respectively. The BL21+MerPBc having a higher affinity for Ni2+ and Cu2+ ions than that of Zn2+ ions was found, whereas BL21+MerPPa preferentially adsorbed Zn2+ ions over Ni2+ and Cu2+ ions. Furthermore, X-ray photoelectron spectroscopy revealed that regardless of the initial ion concentration, Zn, Ni, and Cu were mainly present as oxides in BL21+MerPBc but were mainly present as sulfides at low ion concentrations in BL21+MerPPa, with oxides primarily detected only when the initial ion concentrations were high. These findings were confirmed through X-ray absorption near edge structure and extended X-ray absorption fine structure analyses. These results have revealed that the heavy metal biosorption properties differ between Gram-positive and Gram-negative MerP proteins. |
| 關鍵字 | |
| 語言 | en |
| ISSN | |
| 期刊性質 | 國外 |
| 收錄於 | SCI SSCI Scopus |
| 產學合作 | |
| 通訊作者 | Yi-Huang Hsueh, Kuen-Song Lin |
| 審稿制度 | 是 |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/127464 ) |