期刊論文
| 學年 | 97 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2009-03-01 |
| 作品名稱 | Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture |
| 作品名稱（其他語言） | |
| 著者 | Young, Tai-horng; Lin, Ui-hsiang; Lin, Dar-jong; Chang, Hsu-hsien; 鄭廖平; Cheng, Liao-ping |
| 單位 | 淡江大學化學工程與材料工程學系 |
| 出版者 | Brill Academic Publishers |
| 著錄名稱、卷期、頁數 | Journal of Biomaterials Science 20(5-6), pp.703-720 |
| 摘要 | Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring-opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm<sup>2</sup>. This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine, the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/cm<sup>2</sup>, a value considerably higher than reported in the literature using other immobilization methods. Furthermore, neurons were cultured on L-lysine-immobilized PVDF membranes. The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish. |
| 關鍵字 | POLY(VINYLIDENE FLUORIDE);MEMBRANE;IMMOBILIZATION;L-LYSINE;NEURON;CULTURE |
| 語言 | en |
| ISSN | 0920-5063 |
| 期刊性質 | 國內 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/50495 ) |