期刊論文
| 學年 | 103 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2014-08-08 |
| 作品名稱 | Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators |
| 作品名稱（其他語言） | |
| 著者 | Anh Dzung Nguyen; Huang, Chun-Ching; Liang, Tzu-Wen; Van Bon Nguyen; Pan, Po-Shen; Wang, San-Lang |
| 單位 | 淡江大學化學學系 |
| 出版者 | Kidlington: Pergamon Press |
| 著錄名稱、卷期、頁數 | Carbohydrate Polymers 108, pp.331–337 |
| 摘要 | Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7–9, 60 °C and was stable at pH 7–11, 25–50 °C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains. |
| 關鍵字 | Chitosanase; Squid pen; Penicillium janthinellum; Chitooligomers; Chitosanase activator; Boron |
| 語言 | en |
| ISSN | 0144-8617 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Wang, San-Lang |
| 審稿制度 | 是 |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | ,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/98498 ) |