期刊論文
| 學年 | 102 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2014-01-01 |
| 作品名稱 | An amperometric urea biso sensor based on covalent immobilization of ureaseon N2 incorporated diamond nanowire electrode |
| 作品名稱（其他語言） | |
| 著者 | Jayakumar Shalini; Kamatchi Jothiramalingam Sankaran; Lee, Chi-Young; Tai, Nyan-Hwa; Lin, I-Nan |
| 單位 | 淡江大學物理學系 |
| 出版者 | Amsterdam: Elsevier BV |
| 著錄名稱、卷期、頁數 | Biosensors and Bioelectronics 56, pp.64–70 |
| 摘要 | N2 incorporated diamond nanowire (N-DNW) film electrochemical biosensor has utilized for the quantitative determination of urea in aqueous solution and urine sample. N-DNW electrode is wet-chemically cleaned (oxidation) by boiling in a mixture of H2SO4 and HNO3 (3:1) at 200 °C for 2 h to remove graphite. Urease (Urs) and glutamate dehydrogenase (GLDH) are covalently attached to the oxidized N-DNW electrode by activating the COOH group of N-DNW using ethyl(dimethylaminopropyl)carbodiimide as the coupling agent and N-hydroxysuccinimide as activator. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy data reveal that carboxylic and hydroxyl functionalized nature of N-DNW electrodes Urs–GLDH immobilized N-DNW (Urs–GLDH/N-DNW) has been successfully utilized in urea biosensor which exhibits good performance in sensitivity (6.18 μA/mg dL/cm2), stability (~1 month), reproducibility, lower detection limit (3.87 mg/dL) and fast response time (>10 s). Urs–GLDH/N-DNW also exhibits electrochemical response when tested for different concentration of human urine in buffer solution (from 1:9 to 4:6). In addition, Urs–GLDH/N-DNW bioelectrode retains 80% of its initial enzyme activity for <1 month, when stored at 4–6 °C in a refrigerator. |
| 關鍵字 | N2 incorporated diamond nanowire; Urs–GLDH/N-DNW; Urea; Cyclic voltammetry; Electrochemical impedance spectrsocopy; Amperometry |
| 語言 | en_US |
| ISSN | 0956-5663 1873-4235 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | Lee, Chi-Young |
| 審稿制度 | |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | 紙本 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/97212 ) |