期刊論文
| 學年 | 87 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 1998-10-01 |
| 作品名稱 | Design and characterization of a short HMG-I/DAT1 peptide that binds specifically to the minor groove of DNA |
| 作品名稱（其他語言） | |
| 著者 | 鄭建中; Cheng, Chien-chung; 簡源宏; Jian, Yuan-hung; 羅晴容; Lo, Ching-jung; 程家維; Cheng, Jya-wei |
| 單位 | 淡江大學化學學系 |
| 出版者 | 臺北市：中國化學學會 |
| 著錄名稱、卷期、頁數 | Journal of the Chinese Chemical Society=中國化學會會誌 45(5), pp.619-624 |
| 摘要 | High mobility group protein (HMG) is known to be involved in the formation of high order structure of chromatin. HMGs with minor-groove binding ability in the AT-rich DNA region play a vital role in controlling gene transcription activity. In this report, a 18-residue HMG-I/DAT1 chimeric peptide, PRGRPKGKTLREPRGRPY, was designed and synthesized containing two repetitive PRGRP units and a linking peptide, KGKTLRE, as a targeted DNA-binding peptide. The segment PRGRP is derived from HMG-1 while KGKTLRE is from the DAT1 peptide. Using gel-mobility shift assay and 32P-end labeled 27 bp AT-rich DNA, the dissociation constant of this chimeric peptide was found to he 4.7 × 10^(-6) M, that is, l0^4 times stronger than that of the PRGRP segment stand alone (> 10^(-2) M). In addition, the binding constant was found to increase with the length of AT-rich DNA. The possible DNA binding site of the HMG-I/DAT1 chimeric peptide is determined by footprinting experiments using a minor-groove cleaving agent ruthenium(Ⅲ)-Schiff base complex and a 135-bp 32P-5'-end-labeled DNA restriction fragment of Hind Ⅲ/Rsa I from plasmid pBR322 DNA. The major pattern protected by the HMG-I/DAT1 chimeric peptide exhibits a preference for 5'-AAAT-3' of the AT-rich region. Therefore, this novel design HMG-I/DAT1 chimeric peptide possesses not only a high affinity to AT-rich DNA but also the sequence-specific binding in the minor groove of DNA, which may further lead to the design of short synthetic peptides for therapeutic applications |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0009-4536 |
| 期刊性質 | 國內 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61556 ) |