期刊論文
| 學年 | 95 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2006-11-01 |
| 作品名稱 | Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-Lang; Lin, Tzu-Yin; Yen, Yue-Horng; Liao, Hui-Fen; Chen, Yu-Jen |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Kidlington: Pergamon |
| 著錄名稱、卷期、頁數 | Carbohydrate Research 341(15), pp.2507-2515 |
| 摘要 | |
| 關鍵字 | Antifungal; Antitumor; Chitin oligosaccharides; Chitinase; Bacteria; Bioconversion; Disease control; Enzymes; pH effects; Polysaccharides; Tumors; Wastes; Chitin; chitin; chitinase; unclassified drug; w 118 chitinase; analytic method; article; Bacillus subtilis; biotransformation; chromatography; controlled study; crab; fungal strain; Fusarium oxysporum; high performance liquid chromatography; incubation time; leukemia cell; molecular weight; nonhuman; pH; polymerization; priority journal; shellfish; shrimp; temperature; Animals; Antifungal Agents; Bacillus subtilis; Bacterial Proteins; Cell Division; Chitinase; Crustacea; Humans; Hydrogen-Ion Concentration; K562 Cells; Kinetics; Waste Products; Bacillus subtilis; Decapoda (Crustacea); Fusarium oxysporum |
| 語言 | en |
| ISSN | 0008-6215 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | Bacillus subtilis W-118, a strain that produces antifungal materials, excreted a chitinase when cultured in a medium containing shrimp- and crab-shell powder as the major carbon source. This chitinase, purified by sequential chromatography, had a molecular mass of 20,600 Da and a pI of 6. The optimum pH, optimum temperature, and pH stability of the chitinase were pH 6, 37 °C, and pH 5–7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of Fusarium oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL). The chitinase hydrolyzates of chitin with low degrees of polymerization (DP 1–6) were analyzed by HPLC. Longer reaction times led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on F. oxysporum and human leukemia cell lines. 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18851 ) |