期刊論文
| 學年 | 100 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2012-04-01 |
| 作品名稱 | Taro α-galactosidase: A new gene product for blood conversion |
| 作品名稱（其他語言） | |
| 著者 | Chern, Ming-kai; Li, Huang-yi; Chen, Po-fan; Chien, Su-fang |
| 單位 | 淡江大學化學學系; 淡江大學生命科學研究所 |
| 出版者 | Amsterdam: Elsevier BV |
| 著錄名稱、卷期、頁數 | Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139 |
| 摘要 | The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems. |
| 關鍵字 | Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell |
| 語言 | en |
| ISSN | 1878-8181 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | Chern, Ming-kai; Chien, Su-fang |
| 審稿制度 | |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/76985 ) |