期刊論文
| 學年 | 100 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2011-09-01 |
| 作品名稱 | Electroporation for three commonly used yeast strains for two-hybrid screening experiments |
| 作品名稱（其他語言） | |
| 著者 | Lin, Hsin-ying; Lin, Sey-en; Chien, Su-fang; Chern, Ming-kai |
| 單位 | 淡江大學化學學系 |
| 出版者 | Philadelphia: Elsevier Inc. |
| 著錄名稱、卷期、頁數 | Analytical Biochemistry 416(1), pp.117-119 |
| 摘要 | The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0003-2697 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chern, Ming-kai |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/58243 ) |