期刊論文
| 學年 | 78 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 1990-05-01 |
| 作品名稱 | Isolation and characterization of a novel henegg white lysozyme inihibitor from Bacillus subtilis I-139 |
| 作品名稱（其他語言） | |
| 著者 | Sawao MURAO, Masataka KATO; Wang, San-Lang; Masami Hoshino, Motoo Arai |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| 著錄名稱、卷期、頁數 | Agricultural and Biological Chemistry 54(5), pp.1129-1136 |
| 摘要 | A novel lysozyme inhibitor, hen egg white lysozyme inhibitor (Hewli) was isolated from the culture broth of a bacterium (strain I-139) identified as Bacillus subtilis. Maximum lysozyme inhibitory activity was obtained when the bacterium was grown aerobically in a medium consisting of 0.65 % glucose, 0.5 % sodium glutamate, 1% meat extract, and 4% Polypepton (pH 7.0), at 30°C after 30 to 35hr. Hewli was purified 69-fold from the culture supernatant of B. subtilis I-139 by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephacryl S-200 gel chromatography, DEAE-Toyopearl 650M column chromatography, affinity chromatography on a column of immobilized egg white lysozyme, and acetone extraction. The purified lysozyme inhibitor has an amino acid composition of aspartic acid, glutamic acid, valine, and leucine in a 1:1:1:4 molar ratio and fatty acid composition of palmitic acid and/or vaccenic acid. Hewli inhibited B. subtilis I-139 gh canase and animal lysozymes such as the hen, turkey, or human enzymes. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0002-1369 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | JPN |
| 公開徵稿 | |
| 出版型式 | ,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61859 ) |