期刊論文
| 學年 | 63 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 1975-07-01 |
| 作品名稱 | Presence of endo-β-N-acetylglucosaminidase and protease activities in the commerical neuraminidase preparations isolated from clostridium perfringens |
| 作品名稱（其他語言） | |
| 著者 | Chien, Su-fang; Yevich, Steven J. ; Li, Su-chen; Li,Yu-Teh |
| 單位 | 淡江大學化學學系 |
| 出版者 | Philadelphia, PA: Elsevier Inc. |
| 著錄名稱、卷期、頁數 | Biochemical and biophysical research communications 65(2), pp.683-691 |
| 摘要 | Commercial neuraminidase preparations isolated from Clostridium perfringens were found to be contaminated with endoglycosidase and protease activities. The preparations release oligosaccharide fragment(s) with G1cNAc at the reducing end, from the intact ovalbumin. The enzymes also converted Asn-(GlcNAc)2(Man)5 isolated from ovalbumin into Asn-GlcNAc and an oligosaccharide with G1cNAc at the reducing end. Similar results were obtained when Taka-amylase A and its glycopeptide were used as substrates. These results identify the endoglycosidase activity as endo-β-N-acetylglucosaminidase. Furthermore, the commercial neuraminidase preparations contain a protease activity which hydrolyzes Azocoll, hemoglobin, casein, ovalbumin, human serum albumin, and α1-acid glycoprotein. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0006-291X |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61690 ) |