期刊論文
| 學年 | 97 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2009-05-01 |
| 作品名稱 | Electrophoretic behaviors of human hepatoma HepG2 cells |
| 作品名稱（其他語言） | |
| 著者 | Hsu, Jyh-Ping; Ku, Ming-Hong; Yang, Li-Chia; Lu, Jui-Nan; Young, Tai-Horng H.; Tseng, Shio-Jenn |
| 單位 | 淡江大學數學學系 |
| 出版者 | Weinheim: Wiley - V C H Verlag GmbH & Co. KGaA |
| 著錄名稱、卷期、頁數 | Electrophoresis 30(9), pp.1531-1537 |
| 摘要 | The electrophoretic mobility of HepG2 cells was measured and a charge-regulated model was proposed to simulate the results obtained. Here, a cell was simulated by a rigid core and an ion-penetrable membrane layer containing both acidic and basic functional groups. The influences of the key parameters, including the pH, the ionic strength, the thickness of the membrane layer of a cell, the density and the dissociation constant of the dissociable functional groups in the membrane layer, and the binding constant of divalent cations on the electrophoretic mobility of a cell were investigated. In particular, the role of the buffer used in the experiment was discussed; this effect was neglected in almost all the relevant theoretical analyses in the literature. We showed that the binding ability of divalent cations to the dissociated functional groups in the membrane layer of a cell ranks as Ca2+＞Mg2+＞hexamethonium. |
| 關鍵字 | Buffer effect; Electrophoretic mobility; HepG2 cells; Ion-binding; Surface dissociation reaction |
| 語言 | en |
| ISSN | 1522-2683 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | DEU |
| 公開徵稿 | |
| 出版型式 | 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/58728 ) |