期刊論文
| 學年 | 94 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2006-05-05 |
| 作品名稱 | ShyA, a membrane protein for proper septation of hyphae in Streptomyces |
| 作品名稱（其他語言） | |
| 著者 | Tseng, Shun-fu; Huang, Tzu-wen; Chen, Carton W.; Chern, Ming-kai; Tam, Ming F.; Teng, Shu-chun |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Maryland Heights: Elsevier Inc |
| 著錄名稱、卷期、頁數 | Biochemical and Biophysical Research Communications 343(2), pp.369-377 |
| 摘要 | The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. |
| 關鍵字 | Streptomyces; Actinomycetes; Hyphae; Spore; Membrane protein |
| 語言 | en |
| ISSN | 0006-291X |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Teng, Shu-chun |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/19572 ) |