期刊論文
| 學年 | 97 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2009-05-26 |
| 作品名稱 | Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-lang; Wu, Pei-Chen; Liang, Tzu-wen |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Kidlington: Pergamon |
| 著錄名稱、卷期、頁數 | Carbohydrate Research, 344(8), pp.979-984 |
| 摘要 | We have developed a culture system for efficient production of chitosanase by Bacillus sp. TKU004. TKU004 was cultivated by using squid pen powder as the sole carbon/nitrogen source. The effects of autoclave treatments of the medium on the production of chitosanase were investigated. Autoclave treatment of squid pen powder for 45 min remarkably promoted enzyme productivity. When the culture medium containing an initial squid pen powder concentration of 3% was autoclaved for 45 min, the chitosanase activity was optimal and reached 0.14–0.16 U/mL. In addition, extracellular surfactant-stable chitosanase was purified from the TKU004 culture supernatant. The antioxidant activity of TKU004 culture supernatant was determined through the scavenging ability of DPPH, with 70% per mL. With this method, we have shown that marine wastes can be utilized efficiently through prolonged autoclave treatments to generate a high value-added product, and have revealed its hidden potential in the production of functional foods. |
| 關鍵字 | Chitosanase;Antioxidant;Squid pen wastes;Bacillus sp.;Autoclave treatment |
| 語言 | en |
| ISSN | 0008-6215 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
| 產學合作 | |
| 通訊作者 | Wang, San-lang |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18854 ) |