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化學系 陳曜鴻 教授於
期刊論文
發佈
Molecular structure and developmental expression of zebrafish atp2a genes
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[99-1]
:Molecular structure and developmental expression of zebrafish atp2a genes期刊論文Molecular structure and developmental expression of zebrafish atp2a genesLai, Yen-Yu; Pai, Chiung-Wen; Tsai, I-Ting; Chou, Chi-Yuan; Tsai, Chia-Ti; Chen, Yau-Hung淡江大學化學學系Calcium; Cardiogenesis; Muscle; SERCA; ZebrafishSeoul: Genetics Society of KoreaGenes & Genomics 33(5), pp.541-548We isolated two atp2a genes, atp2a1 and atp2a2a, from embryonic zebrafish. Amino acid sequences deduced from zebrafish atp2a genes are aligned with orthologue proteins from other species, the results showed that they share high percentage of identities (82%–94%) and acidic pIs (5.03–5.33). Whole mount in situ hybridization experiments showed that atp2a1 and atp2a2a are maternal inherited genes which can be detected at 1-cell stage embryos and express in the entire animal pole from 6 hours post-fertilization (hpf) to 12 hpf. At the later stages (48–96 hpf), expression of atp2a1 was restricted in head and trunk muscles as well as in
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