教師資料查詢 | 類別: 期刊論文 | 教師: 廖康伶 KANG-LING LIAO (瀏覽個人網頁)

標題:A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
學年105
學期2
出版(發表)日期2017/04/14
作品名稱A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
作品名稱(其他語言)
著者2. Liang Ying, Daisuke Urano, Kang-Ling Liao, Tyson L Hedrick, Yajun Gao, Alan M Jones
單位
出版者
著錄名稱、卷期、頁數Plant Methods. 2017; 13: 26.
摘要Background
Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. However, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes.

Results
We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios.

Conclusion
This method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. This method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.
關鍵字Arabidopsis thaliana; C/N sensing; Chlorophyll content; ColorChecker chart; Heterotrimeric G protein complex; Stress assay
語言英文(美國)
ISSN
期刊性質國外
收錄於SCI;
產學合作
通訊作者Alan M Jones
審稿制度
國別美國
公開徵稿
出版型式,電子版
相關連結
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