Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers (IBN-4) in Prodrug-Activated Cancer Theranostics
學年 104
學期 1
出版（發表）日期 2015-12-04
作品名稱 Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers (IBN-4) in Prodrug-Activated Cancer Theranostics
作品名稱（其他語言）
著者 Hung-BY; Kuthati-Y; Kankala-RK; Kankala-S; Deng-JP; Liu-CL; Lee CH
單位
出版者
著錄名稱、卷期、頁數 Nanomaterials 5, pp.2169-2191
摘要 To develop a carrier for use in enzyme prodrug therapy, Horseradish peroxidase (HRP) was immobilized onto mesoporous silica nanoparticles (IBN-4: Institute of Bioengineering and Nanotechnology), where the nanoparticle surfaces were functionalized with 3-aminopropyltrimethoxysilane and further conjugated with glutaraldehyde. Consequently, the enzymes could be stabilized in nanochannels through the formation of covalent imine bonds. This strategy was used to protect HRP from immune exclusion, degradation and denaturation under biological conditions. Furthermore, immobilization of HRP in the nanochannels of IBN-4 nanomaterials exhibited good functional stability upon repetitive use and long-term storage (60 days) at 4 °C. The generation of functionalized and HRP-immobilized nanomaterials was further verified using various characterization techniques. The possibility of using HRP-encapsulated IBN-4 materials in prodrug cancer therapy was also demonstrated by evaluating their ability to convert a prodrug (indole-3-acetic acid (IAA)) into cytotoxic radicals, which triggered tumor cell apoptosis in human colon carcinoma (HT-29 cell line) cells. A lactate dehydrogenase (LDH) assay revealed that cells could be exposed to the IBN-4 nanocomposites without damaging their membranes, confirming apoptotic cell death. In summary, we demonstrated the potential of utilizing large porous mesoporous silica nanomaterials (IBN-4) as enzyme carriers for prodrug therapy.
關鍵字 mesoporous silica nanoparticles;enzyme immobilization;Institute of Bioengineering and Nanotechnology (IBN-4);anti-cancer;prodrug therapy
語言 en
ISSN 2079-4991
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chia-Hung Lee
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
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