教師資料查詢 | 類別: 期刊論文 | 教師: 謝忠宏 HSIEH, CHUNG-HUNG (瀏覽個人網頁)

標題:Nitric Oxide Physiological Responses and Delivery Mechanisms Probed by Water-Soluble Roussin’s Red Ester and {Fe(NO)2}10 DNIC
學年97
學期1
出版(發表)日期2008/08/01
作品名稱Nitric Oxide Physiological Responses and Delivery Mechanisms Probed by Water-Soluble Roussin’s Red Ester and {Fe(NO)2}10 DNIC
作品名稱(其他語言)
著者Chen, Yi-Ju; Ku, Wei-Chi; Feng, Li-Ting; Tsai, Ming-Li; Hsieh, Chung-Hung; Hsu, Wen-Hwei; Liaw, Wen-Feng; Hung, Chen-Hsiung; Chen, Yu-Ju
單位淡江大學化學學系
出版者Washington, DC: American Chemical Society
著錄名稱、卷期、頁數Journal of the American Chemical Society 130(33), pp.10929-10938
摘要Dinitrosyl−iron complexes (DNICs) are stable carriers for nitric oxide (NO), an important biological signaling molecule and regulator. However, the insolubility of synthetic DNICs, such as Roussin’s red ester (RRE), in water has impaired efforts to unravel their biological functions. Here, we report a water-soluble and structurally well-characterized RRE [Fe(μ-SC2H4COOH)(NO)2]2 (DNIC-1) and a {Fe(NO)2}10 DNIC [(PPh2(Ph-3-SO3Na))2Fe(NO)2] (DNIC-2), their NO-induced protein regulation, and their cellular uptake mechanism using immortalized vascular endothelial cells as a model. Compared with the most common NO donor, S-nitroso-N-acetyl-penicillamine (SNAP), the in vitro NO release assay showed that both DNICs acted as much slower yet higher stoichiometric NO-release agents with low cytotoxicity (IC50 > 1 mM). Furthermore, l-cysteine facilitated NO release from SNAP and DNIC-1, but not DNIC-2, in a dose- and time-dependent manner. EPR spectroscopic analysis showed, for the first time, that intact DNIC-1 can either diffuse or be transported into cells independently and can transform to either paramagnetic protein bound DNIC in the presence of serum or [DNIC-(Cys)2] with excess l-cysteine under serum-free conditions. Both DNICs subsequently induced NO-dependent upregulation of cellular heat shock protein 70 and in vivo protein S-nitrosylation. We conclude that both novel water-soluble DNICs have potential to release physiologically relevant quantities of NO and can be a good model for deciphering how iron−sulfur−nitrosyl compounds permeate into the cell membrane and for elucidating their physiological significance.
關鍵字
語言英文
ISSN0002-7863;1520-5126
期刊性質國外
收錄於
產學合作
通訊作者
審稿制度
國別美國
公開徵稿
出版型式紙本;電子版
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