教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Microbial reclamation of shellfish wastes for the production of chitinases
學年89
學期2
出版(發表)日期2001/03/01
作品名稱Microbial reclamation of shellfish wastes for the production of chitinases
作品名稱(其他語言)
著者Wang, San-lang; Hwang, Jau-ren
單位淡江大學生命科學研究所
出版者PA: Elsevier Inc.
著錄名稱、卷期、頁數Enzyme and Microbial Technology 28(4-5), pp.376-382
摘要Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50°C, pH 7 to 10, and 70°C; those of FB2 were pH 9, 60°C, pH 5 to 9, and 70°C; and those of FB3 were pH 7, 50°C, pH 5 to 9, and 60°C. The activities of all enzymes were strongly inhibited by Hg2+ and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol.
關鍵字Shrimp shell; Chitin; Chitinase; Bacillus cereus; Bacillus alvei; Bacillus sphaericus
語言英文
ISSN0141-0229
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者
審稿制度
國別
公開徵稿
出版型式
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