教師資料查詢 | 類別: 期刊論文 | 教師: 陳銘凱 CHERN MING-KAI (瀏覽個人網頁)

標題:Electroporation for three commonly used yeast strains for two-hybrid screening experiments
學年100
學期1
出版(發表)日期2011/09/01
作品名稱Electroporation for three commonly used yeast strains for two-hybrid screening experiments
作品名稱(其他語言)
著者Lin, Hsin-ying; Lin, Sey-en; Chien, Su-fang; Chern, Ming-kai
單位淡江大學化學學系
出版者Philadelphia: Elsevier Inc.
著錄名稱、卷期、頁數Analytical Biochemistry 416(1), pp.117-119
摘要The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
關鍵字
語言英文
ISSN0003-2697
期刊性質國外
收錄於SCI
產學合作
通訊作者Chern, Ming-kai
審稿制度
國別美國
公開徵稿
出版型式紙本
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