教師資料查詢 | 類別: 期刊論文 | 教師: 周子聰 Zicong Zhou (瀏覽個人網頁)

標題:Identification of Peptides Using Gold Nanoparticle-Assisted Single-Drop Microextraction Coupled with AP-MALDI Mass Spectrometry
學年94
學期1
出版(發表)日期2005/10/05
作品名稱Identification of Peptides Using Gold Nanoparticle-Assisted Single-Drop Microextraction Coupled with AP-MALDI Mass Spectrometry
作品名稱(其他語言)
著者Sudhir, Putty-reddy; Wu, Hui-fen; 周子聰; Zhou, Zi-cong
單位淡江大學物理學系
出版者American Chemical Society (ACS)
著錄名稱、卷期、頁數Analytical Chemistry 77(22), pp. 7380-7385
摘要A novel technique, gold nanoparticle-assisted single-drop microextraction (SDME) combined with atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) for the identification of peptides has been described. The SDME of peptides from aqueous solution was achieved using gold nanoparticles prepared in toluene as the acceptor phase. A simple phenomenon of isoelectric point (pI) of the peptides has been utilized successfully to extract the peptides into a single drop of nanogold in toluene. After extraction, a single-drop nano gold solution was directly spotted onto the target plate with an equal volume of matrix, ∝-cyanohydroxy cinnamic acid (∝-CHCA) and analyzed in AP-MALDI-MS. The parameters, such as solvent selection, extraction time, agitation rate, and pH effect, were optimized for the SDME technique. Using this technique, in aqueous solution, the lowest concentration detected for Met- and Leu-enkephalin peptides was 0.2 and 0.17 μM, respectively. In addition, the application of this technique to obtain the signal for the selected peptides in a mass spectrum in the presence of matrix interferences such as 1% Triton X-100 and 6.5 M urea has been showed. The application was extended to identify the peptides spiked into urine.
關鍵字
語言英文
ISSN0003-2700
期刊性質國內
收錄於
產學合作
通訊作者
審稿制度
國別中華民國
公開徵稿
出版型式,電子版
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