A 23-Amino Acid Motif Spanning the Basic Domain Targets Zebrafish Myogenic Regulatory Factor Myf5 into Nucleolus
學年 94
學期 1
出版(發表)日期 2005-10-01
作品名稱 A 23-Amino Acid Motif Spanning the Basic Domain Targets Zebrafish Myogenic Regulatory Factor Myf5 into Nucleolus
作品名稱(其他語言)
著者 陳曜鴻; Chen, Yau-hung; Wang, Y. H.; Lu, C. H.; Tsai, H. J.
單位 淡江大學生命科學研究所
出版者 Mary Ann Liebert
著錄名稱、卷期、頁數 DNA and cell biology 24(10), pp.651-660
摘要 Myf5 is a nuclear protein and one of the basic helix-loop-helix (bHLH) myogenic factors that play an important role in muscle specification and differentiation. The motif responsible for the nuclear translocation of Myf5 was unknown. Using on-line monitoring of EGFP (enhanced green fluorescent protein)-tagged zebrafish Myf5 translocation, we demonstrated that Myf5–EGFP protein resided in the nucleoplasm and nucleolus of zebrafish fibroblast cell lines (ZEM2S and ZF4), mammalian nonmuscle cell line (COS1), and muscle cell lines (RD and C2C12). In contrast, zebrafish MyoD–EGFP was localized in the nucleus but did not condense in the nucleolus. Using indirect immunofluorescent staining, we determined that zebrafish Myf5 was colocalized with nucleophosmin/B23, a nucleolus protein. Deletion analysis revealed that amino acid residues 60 to 82 (60KRKASTVDRRRAATMRERRRLKK82) of Myf5 were sufficient and necessary for nucleolus targeting. A GST pulldown assay followed by Western analysis showed that nucleolin/C23 could be pulled down specifically by GST–Myf5, but not by GST–MyoD. Based on these findings, we propose that the distinct functions of Myf5 and MyoD may result from their differential binding affinity to nucleolin/C23.
關鍵字
語言 en_US
ISSN 1044-5498
期刊性質 國外
收錄於
產學合作
通訊作者
審稿制度
國別 USA
公開徵稿
出版型式 ,紙本
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