期刊論文

學年 110
學期 2
出版(發表)日期 2022-07-05
作品名稱 Potential of the liquid fermentation of fishery waste by Paenibacillus elgii for metalloprotease production
作品名稱(其他語言)
著者 Chien Thang Doan; Thi Ngoc Tran; Minh Trung Nguyen; Huu Kien Nguyen; Thi Kim Thi Tran; Thi Hanh Nguyen; Thi Phuong Hanh Tran; Van Bon Nguyen; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Polymers 14(13), 2741
摘要 This study attempted to use fishery processing wastes to produce protease by Paenibacillus elgii TKU051. Of the tested wastes, tuna head powder (THP) was found to be the most effective carbon and nitrogen (C/N) source, and the optimal conditions were as follows: 0.811% THP, 0.052% K2HPO4 , 0.073% MgSO4 , initial pH of 8.96, incubation temperature of 31.4 ◦C, and incubation time of 3.092 days to achieve the maximum protease activity of 2.635 ± 0.124 U/mL. A protease with a molecular weight of 29 kDa was purified and biochemically characterized. Liquid chromatography with tandem mass spectrometry analysis revealed an amino acid sequence of STVHYSTR of P. elgii TKU051 protease, suggesting that the enzyme may belong to the M4 family of metalloproteases. The optimal activity of the enzyme was achieved at 60 ◦C and pH 8. P. elgii TKU051 protease was strongly inhibited by ethylenediaminetetraacetic acid and 1,10-phenanthroline, indicating its precise metalloprotease property. P. elgii TKU051 protease displayed the activity toward casein and raw fishery wastes such as tuna heads, tuna viscera, shrimp heads, and squid pens. Finally, the purified P. elgii TKU051 protease could improve the free-radical scavenging activity of fishery wastes. In short, P. elgii TKU051 has potential application in eco-friendly approaches to efficiently convert fishery wastes to metalloprotease.
關鍵字 antioxidant;metalloprotease;fishery waste;Paenibacillus elgii;protease;tuna heads
語言 en
ISSN 2073-4360
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 WANG San-Lang
審稿制度
國別 CHE
公開徵稿
出版型式 ,電子版