期刊論文
| 學年 | 110 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2022-01-17 |
| 作品名稱 | Protective role of Bu-Zhong-Yi-Qi decoction on aristolochic acid-intoxicated zebrafish |
| 作品名稱（其他語言） | |
| 著者 | Tsai JN; Ding YJ; Lo KC; Lu PH; Chang CF; Wen CC; Wang BC; Yang LL; Chen YH |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Tropical Journal of Pharmaceutical Research 20(3), p.605-611 |
| 摘要 | Purpose: To investigate the potential nephroprotective effects of a traditional Chinese medical prescription, Bu-Zhong-Yi-Qi Decoction (BZYQD) in an aristolochic acid (AA)-intoxicated zebrafish model. Methods: A green fluorescent zebrafish line Tg (wt1b:EGFP) was used, and different exposure protocols were applied. Once a suitable protective concentration of BZYQD was found, antibody staining and real-time PCR methods were applied and the pro-inflammatory gene expressions were determined. Results: The results showed that low dose (10 ppm) of BZYQD attenuates the AA-induced malformed kidney phenotype. This finding was further substantiated by an examination of the integrity of pronephric tubes in the treated embryos. Pre-treatment with BZYQD suppressed the elevated expressions of pro- inflammatory genes, including tumor necrosis factor-α and myeloperoxidase, induced by AA exposure (1.6 ~ 2.3-fold). This indicates that the nephroprotective effect of BZYQD may be mediated by suppression of pro-inflammatory gene expression. On the other hand, a high dose (500 ppm) of BZYQD caused nephrotoxic effect. Conclusion: An efficient model to identify AA-protective compounds using zebrafish embryos has been successfully established. Using this strategy, it has been found that BZYQD is nephroprotective in zebrafish embryos, and might have the potential to be applied in humans. |
| 關鍵字 | Bu-Zhong-Yi-Qi Decoction;Zebrafish;Kidney;Aristolochic acid;Nephroprotection |
| 語言 | en_US |
| ISSN | 1596-5996 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | NGA |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/122352 ) |