期刊論文

標題 Conversion of pectin-containing by-products to pectinases by Bacillus amyloliquefaciens and its applications on hydrolyzing banana peels for prebiotics production
學年 109
學期 2
出版(發表)日期 2021/05/04
作品名稱 Conversion of pectin-containing by-products to pectinases by Bacillus amyloliquefaciens and its applications on hydrolyzing banana peels for prebiotics production
作品名稱(其他語言)
著者 Doan CT; Chen CL; Nguyen VB; Tran TN; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數 Polymers 13(9), 1483
摘要 The utilization of pectin-containing by-products may be useful in a variety of fields. This study aims to establish the processing of pectin-containing by-products to produce pectinases using Bacillus amyloliquefaciens TKU050 strain. In this study, several kinds of agricultural pectin-containing by-products from banana (banana peel), rice (rice bran), orange (orange peel), coffee (spent coffee grounds), and wheat (wheat bran) were utilized to provide carbon sources for the production of a pectinase by B. amyloliquefaciens TKU050. B. amyloliquefaciens TKU050 expressed the highest pectinase productivity (0.76 U/mL) on 0.5% wheat bran-containing medium at 37 °C for four days. A 58 kDa pectinase was purified from the four-day cultured medium fermented under optimized culture conditions with 7.24% of a recovery ratio and 0.51 U/mg of specific activity, respectively. The optimum temperature, optimum pH, thermal stability, and pH stability of the TKU050 pectinase were 50 °C, pH 6, <50 °C, and pH 6–9, respectively. The TKU050 pectinase was inhibited by sodium dodecyl sulfate and Cu2+. The reducing sugar obtained by hydrolyzing banana peel with TKU050 pectinase showed the growth-enhancing effect on the growth of four tested lactic acid
關鍵字 pectin;pectinase;wheat bran;banana peel;Bacillus amyloliquefaciens;prebiotics
語言 英文
ISSN 2073-4360
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 San-Lang, Wang
審稿制度
國別 瑞士
公開徵稿
出版型式 ,電子版