期刊論文

標題 Conversion of wheat bran to xylanases and dye adsorbent by Streptomyces thermocarboxydus
學年 109
學期 1
出版(發表)日期 2021/01/17
作品名稱 Conversion of wheat bran to xylanases and dye adsorbent by Streptomyces thermocarboxydus
作品名稱(其他語言)
著者 Tran TN; Doan CT; Wang SL
單位
出版者
著錄名稱、卷期、頁數 Polymers 13(2), 287
摘要 Agro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by Streptomyces thermocarboxydus TKU045. The optimal media for xylanase production was 2% (w/v) WBP, 1.50% (w/v) KNO3, 0.05% (w/v) MgSO4, and 0.10% (w/v) K2HPO4, and the optimal culture conditions were 50 mL (in a 250 mL-volume Erlenmeyer flask), initial pH 9.0, 37 °C, 125 rpm, and 48 h. Accordingly, the highest xylanase activity was 6.393 ± 0.130 U/mL, 6.9-fold higher than that from un-optimized conditions. S. thermocarboxydus TKU045 secreted at least four xylanases with the molecular weights of >180, 36, 29, and 27 kDa when cultured on the WBP-containing medium. The enzyme cocktail produced by S. thermocarboxydus TKU045 was optimally active over a broad range of temperature and pH (40–70 °C and pH 5–8, respectively) and could hydrolyze birchwood xylan to produce xylobiose as the major product. The obtained xylose oligosaccharide (XOS) were investigated for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the growth effect of lactic acid bacteria. Finally, the solid waste from the WBP fermentation using S. thermocarboxydus TKU045 revealed the high adsorption of Congo red, Red 7, and Methyl blue. Thus, S. thermocarboxydus TKU045 could be a potential strain to utilize wheat bran to produce xylanases for XOS preparation and dye adsorbent.
關鍵字 Agro-byproducts;dye adsorption;antioxidant;prebiotic;Streptomyces thermocarboxydus;xylanase;xylo-oligosaccharide
語言 英文
ISSN 2073-4360
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 San-Lang Wang
審稿制度
國別 瑞士
公開徵稿
出版型式 ,電子版