期刊論文

標題 Utilization of seafood processing by-products for production of proteases by Paenibacillus sp. TKU052 and their application in biopeptides' preparation
學年 109
學期 1
出版(發表)日期 2020/11/20
作品名稱 Utilization of seafood processing by-products for production of proteases by Paenibacillus sp. TKU052 and their application in biopeptides' preparation
作品名稱(其他語言)
著者 Chien Thang Doan;Thi Ngoc Tran;Van Bon Nguyen;Anh Dzung Nguyen;San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Marine Drugs 18(11), 574
摘要 Microbial fermentation of by-products is a renewable and efficient technique in the development of a range of useful products. In this study, protease synthesis by Paenibacillus sp. TKU052 was carried out on culture media containing some common seafood processing by-products (SPBPs) as the sole source of carbon and nitrogen (C/N). The most suitable C/N nutrition source for the production of proteases was found to be 3.0% (w/v) demineralized crab shells powder (deCSP) and maximal enzyme activity of 4.41 ± 0.16 U/mL was detected on the third day of the culture. Two proteases (P1 and P2) with a similar molecular weight of 31 kDa were successfully isolated and purified from the 3-day deCSP-containing medium. Both P1 and P2 exhibited the highest activity of gelatin hydrolysis at pH 6 and 60 °C. The gelatin hydrolysates catalyzed by Paenibacillus TKU052 proteases were evaluated for biological activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, angiotensin-I converting enzyme (ACE) inhibition, and prebiotic activities. The gelatin hydrolysates expressed 31.76–43.95% DPPH radical scavenging activity and 31.58–36.84% ACE inhibitory activity, which was higher than those from gelatin. Gelatin hydrolysates also showed the growth-enhancing effect on Bifidobacterium bifidum BCRC 14615 with an increase to 135.70–147.81%. In short, Paenibacillus sp. TKU052 could be a potential strain to utilize crab shell wastes to produce proteases for bio-active peptides’ preparation.
關鍵字 angiotensin-I converting enzyme inhibitory activity;free radical scavenging activity;Paenibacillus;peptide;prebiotic;protease
語言 英文
ISSN
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 San-Lang Wang
審稿制度
國別 瑞士
公開徵稿
出版型式 ,電子版