期刊論文
| 學年 | 104 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2015-12-23 |
| 作品名稱 | Intrinsic coordination for revealing local structural changes in protein folding-unfolding |
| 作品名稱（其他語言） | |
| 著者 | Shiu, Ying-Jen; Hayashi, Michitoshi; Shih, Orion; Su, Charlene; Tsai, Min-Yeh; Yeh, Yi-Qi; Su, Chun-Jen; Huang, Yu-Shan; Lin, Sheng-Hsien; Jeng, U-Ser |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Physical Chemistry Chemical Physics, 18(4), 3179 |
| 摘要 | With a deformed object of a rigid rod inside, the local dislocations may be tracked relatively easily with respect to the internal rigid rod. We apply this concept on protein folding-unfolding to track the internal structural changes of an unfolded protein in solution. Proposed here is a protein internal coordination based on the major axis X of an ellipsoidal protein and the stable intrinsic transition dipole moment μ of the protein during unfolding. In this methodology, small-angle X-ray scattering (SAXS) is used to provide the protein global morphologies in the native and unfolded states. Furthermore, time-resolved fluorescence anisotropy (TRFA) provides the relative orientation between X and μ of Trp59 of the model protein cytochrome c. Hence observed in the protein unfolding with denaturants, acid, urea, or GuHCl, is the elongation of the native protein conformation along a reoriented protein major axis; accompanied are the different extents of relocations of the terminal α helices and loop structures of the protein in the corresponding unfolding. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 1463-9076 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/117143 ) |