期刊論文

標題 Anti-α-glucosidase activity by a protease from Bacillus licheniformis
學年 107
學期 2
出版(發表)日期 2019/02/15
作品名稱 Anti-α-glucosidase activity by a protease from Bacillus licheniformis
作品名稱(其他語言)
著者 Chien Thang Doan; Thi Ngoc Tran; Minh Trung Nguyen; Van Bon Nguyen; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Molecules 24(4), 691
摘要 Anti-α-glucosidase (AAG) compounds have received great attention due to their potential use in treating diabetes. In this study, Bacillus licheniformis TKU004, an isolated bacterial strain from Taiwanese soil, produced AAG activity in the culture supernatant when squid pens were used as the sole carbon/nitrogen (C/N) source. The protein TKU004P, which was isolated from B. licheniformis TKU004, showed stronger AAG activity than acarbose, a commercial anti-diabetic drug (IC50 = 0.1 mg/mL and 2.02 mg/mL, respectively). The molecular weight of TKU004P, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa. High-performance liquid chromatography (HPLC) analysis showed that TKU004P may be a protease that demonstrates AAG activity by degrading yeast α-glucosidase. Among the four chitinous sources of C/N, TKU004P produced the highest AAG activity in the culture supernatant when shrimp head powder was used as the sole source (470.66 U/mL). For comparison, 16 proteases, were investigated for AAG activity but TKU004P produced the highest levels. Overall, the findings suggest that TKU004P could have applications in the biochemical and medicinal fields thanks to its ability to control the activity of α-glucosidase.
關鍵字 anti-α-glucosidase;protease;diabetes;microbial conversion;Bacillus licheniformis
語言 英文
ISSN 1420-3049
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 WANG San-Lang
審稿制度
國別 瑞士
公開徵稿
出版型式 ,電子版