期刊論文

標題 Conversion of squid pens to chitosanases and proteases via Paenibacillus sp TKU042
學年 106
學期 2
出版(發表)日期 2018/03/06
作品名稱 Conversion of squid pens to chitosanases and proteases via Paenibacillus sp TKU042
作品名稱(其他語言)
著者 Doan CT; Tran TN; Nguyen VB; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數 Marine Drugs 16(83), pp.1-12
摘要 Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources.
關鍵字 Paenibacillus;chitin;chitosan;chitosanase;protease;squid pens;α-glucosidase inhibitors
語言 英文
ISSN 1660-3397
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 WANG San-Lang
審稿制度
國別 中華民國
公開徵稿
出版型式 ,電子版