標題
|
Conversion of shrimp heads to α-glucosidase inhibitors via co-culture of Bacillus mycoides TKU040 and Rhizobium sp. TKU041 |
學年
|
107 |
學期
|
1 |
出版(發表)日期
|
2018/08/01 |
作品名稱
|
Conversion of shrimp heads to α-glucosidase inhibitors via co-culture of Bacillus mycoides TKU040 and Rhizobium sp. TKU041 |
作品名稱(其他語言)
|
|
著者
|
Hsu C.H.; Nguyen V.B.; Nguyen A.D.; Wang San-Lang |
單位
|
|
出版者
|
|
著錄名稱、卷期、頁數
|
Research on Chemical Intermediates 44(8), p.4597–4607 |
摘要
|
Alpha-glucosidase inhibitors (aGIs) have potential use as antidiabetic drugs for the treatment of type II diabetes. Most aGIs place a burden on the liver and cause gastrointestinal distress, therefore the development of new aGIs has become very important. In this study, we investigated the production of aGIs by the co-culture of Bacillus mycoides TKU040 and Rhizobium sp. TKU041 using shrimp head powder (SHP) as the sole source of carbon and nitrogen. After fermentation in 50 mL of 1% SHP-containing medium (0.1% K2HPO4 and 0.05% MgSO4·7H2O, pH 9.2) at 37 °C for 4 days, the maximum productivity of aGIs (143 U/mL) was reached. The IC50 of the aGIs produced in the culture supernatant was 3 mg/mL. The aGI activity was only 60% after treatment at pH 3 for 30 min; this increased to 140% after treatment at pH 11 for 30 min. The aGI activity remained at 60% after treatment at 60 °C for 30 min. |
關鍵字
|
diabetes;glucosidase inhibitors;Bacillus;Rhizobium |
語言
|
英文 |
ISSN
|
0922-6168;1568-5675 |
期刊性質
|
國外 |
收錄於
|
SCI; |
產學合作
|
|
通訊作者
|
Wang San-Lang |
審稿制度
|
是 |
國別
|
美國 |
公開徵稿
|
|
出版型式
|
,電子版 |