期刊論文

標題 Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015
學年 104
學期 2
出版(發表)日期 2016/03/22
作品名稱 Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015
作品名稱(其他語言)
著者 Tzu-Wen Liang; Shan-Ni Jen; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Polymers 8(3), pp.98(11 pages)
摘要 The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.
關鍵字 PLA;squid pen;Pseudomonas tamsuii;PLA depolymerase;degradation
語言 英文
ISSN 2073-4360
期刊性質 國外
收錄於 SCI;
產學合作
通訊作者 San-Lang Wang
審稿制度
國別 瑞士
公開徵稿
出版型式 ,電子版