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標題
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Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015 |
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學年
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104 |
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學期
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2 |
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出版（發表）日期
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2016/03/22 |
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作品名稱
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Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015 |
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作品名稱（其他語言）
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著者
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Tzu-Wen Liang; Shan-Ni Jen; Anh Dzung Nguyen; San-Lang Wang |
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單位
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出版者
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著錄名稱、卷期、頁數
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Polymers 8(3), pp.98(11 pages) |
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摘要
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The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources. |
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關鍵字
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PLA;squid pen;Pseudomonas tamsuii;PLA depolymerase;degradation |
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語言
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英文 |
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ISSN
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2073-4360 |
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期刊性質
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國外 |
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收錄於
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SCI; |
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產學合作
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通訊作者
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San-Lang Wang |
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審稿制度
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是 |
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國別
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瑞士 |
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公開徵稿
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出版型式
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,電子版 |