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標題
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Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators |
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學年
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103 |
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學期
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1 |
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出版（發表）日期
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2014/08/08 |
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作品名稱
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Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators |
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作品名稱（其他語言）
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著者
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Anh Dzung Nguyen; Huang, Chun-Ching; Liang, Tzu-Wen; Van Bon Nguyen; Pan, Po-Shen; Wang, San-Lang |
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單位
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淡江大學化學學系 |
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出版者
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Kidlington: Pergamon Press |
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著錄名稱、卷期、頁數
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Carbohydrate Polymers 108, pp.331–337 |
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摘要
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Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7–9, 60 °C and was stable at pH 7–11, 25–50 °C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains. |
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關鍵字
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Chitosanase; Squid pen; Penicillium janthinellum; Chitooligomers; Chitosanase activator; Boron |
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語言
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英文 |
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ISSN
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0144-8617 |
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期刊性質
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國外 |
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收錄於
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SCI; |
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產學合作
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通訊作者
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Wang, San-Lang |
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審稿制度
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是 |
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國別
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英國 |
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公開徵稿
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出版型式
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,紙本 |