期刊論文
| 學年 | 90 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2002-04-01 |
| 作品名稱 | Expression, purification and DNA-binding activity of tilapia muscle-specific transcription factor, MyoD, produced in Escherichia coli |
| 作品名稱（其他語言） | |
| 著者 | Chen, Yau-Hung; Liang, Chin-Tien ; Tsai, Huai-Jen |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Philadelphia: Elsevier Inc. |
| 著錄名稱、卷期、頁數 | Comparative Biochemistry and Physiology Part B 131(4), pp.795-805 |
| 摘要 | MyoD is one of several helix-loop-helix proteins regulating muscle-specific gene expression. Using a reverse transcription-polymerase chain reaction, 5′-rapid cDNA end amplification, and plaque hybridization, MyoD cDNA was cloned from the mRNA of tilapia dorsal skeletal muscle. The 1015 bp MyoD cDNA product contained an 846 bp open reading frame with flanking regions of 115 and 64 bp at the 5′- and 3′-ends, respectively. Results showed that the tilapia MyoD sequence, which includes one polypeptide of 281 amino acids, shared sequence identities of 64.3, 64.1, 62.6 and 62.4% with those of zebrafish, carp, and two rainbow trout, respectively. Results from a molecular phylogenic tree assay showed that the tilapia MyoD was more closely related to those of other fishes than of higher vertebrates. Using Escherichia coli, a pET expression system, and an Ni2+-NTA column, we purified ∼35 kDa recombinant tilapia MyoD. Results from an electrophoretic mobility shift assay demonstrated that the purified E. coli-produced tilapia MyoD was capable of binding to the DNA fragment sequence CA(C/T)(C/A)TG. © 2002 Elsevier Science Inc. All rights reserved. |
| 關鍵字 | cDNA;Fish; MyoD;Ni2+-NTA column;pET expression system;Phylogenic tree;Plaque hybridization;Tilapia |
| 語言 | en |
| ISSN | 1096-4959 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Tsai, H. J. |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61853 ) |