期刊論文

標題 Purification and characterization of two bifunctional chitinases/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium
學年 85
學期 2
出版(發表)日期 1997/02/01
作品名稱 Purification and characterization of two bifunctional chitinases/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium
作品名稱(其他語言)
著者 Wang, San-Lang; Chang, Wen-Tsu
單位 淡江大學生命科學研究所
出版者 Washington: American Society for Microbiology
著錄名稱、卷期、頁數 Applied and Environmental Microbiology 63(2), pp.380-386
摘要 Two extracellular chitinases (FI and FII) were purified from the culture supernatant of Pseudomonas aeruginosa K-187. The molecular weights of FI and FII were 30,000 and 32,000, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 60,000 and 30,000, respectively, by gel filtration. The pIs for FI and FII were 5.2 and 4.8, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FI were pH 8, 50 degrees C, pH 6 to 9, and 50 degrees C; those of FII were pH 7, 40 degrees C, pH 5 to 10, and 60 degrees C. The activities of both enzymes were activated by Cu2+; strongly inhibited by Mn2+, Mg2+, and Zn2+; and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol. Both chitinases showed lysozyme activity. The purified enzymes had antibacterial and cell lysis activities with many kinds of bacteria. This is the first report of a bifunctional chitinase/lysozyme from a prokaryote.
關鍵字
語言 英文
ISSN 0099-2240
期刊性質 國外
收錄於 SCI;EI
產學合作
通訊作者 Wang, San-Lang
審稿制度
國別 美國
公開徵稿
出版型式 紙本