會議論文
| 學年 | 95 |
|---|---|
| 學期 | 1 |
| 發表日期 | 2007-05-25 |
| 作品名稱 | Bioconversion of Shellfish Chitin Wastes for the Production of Bacillus subtilis W-118 Chitinase |
| 作品名稱（其他語言） | |
| 著者 | 王三郎; Wang, San-Lang; Lin, Tzu-Yin; Yen, Yue-Horng |
| 作品所屬單位 | 淡江大學生命科學研究所 |
| 出版者 | |
| 會議名稱 | 2007年 台灣幾丁質幾丁聚醣研討會=2007 Taiwan Chitin and Chitosan Symposium |
| 會議地點 | 中壢, 臺灣 |
| 摘要 | Bacillus subtilis W-118, an antifungal materials producing strain, excreted one chitinase when cultured in a medium containing shrimp and crab shell powder as major carbon source. The chitinase, purified by sequential chromatography, had a molecular mass of 20,600 and pI of 6. The optimum pH, optimum temperature, and pH stability of the chitinase were pH 6, 37.degree.C, and pH 5-7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of F. oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL).The chitinase hydrolyzates of chitin with low degrees of polymerization (DP) from 1 to 6 were analyzed by HPLC. Longer reaction time led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on Fusarium oxysporum and human leukemia cell lines. |
| 關鍵字 | |
| 語言 | zh_TW |
| 收錄於 | |
| 會議性質 | 國內 |
| 校內研討會地點 | |
| 研討會時間 | 20070525~20070525 |
| 通訊作者 | |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | |
| 出處 | 2007年 台灣幾丁質幾丁聚醣研討會論文集=Proceedings of 2007 Taiwan Chitin and Chitosan Symposium, 2p. |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/85550 ) |